Molecular identification of Trichinella species by multiplex PCR: new insight for Trichinella murrelli
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چکیده
منابع مشابه
Molecular identification of Trichinella species by multiplex PCR: new insight for Trichinella murrelli
In order to identify Trichinella at the species level, the commonly used test is a multiplex PCR, allowing the discrimination of nine out of the twelve taxa described so far. This test is based on five primer pairs amplifying fragments of the large subunit rDNA. Each taxon produces one or two bands of different sizes, resulting in a specific band pattern. By multiplex PCR, Trichinella murrelli ...
متن کاملA single, multiplex PCR for differentiating all species of Trichinella.
The genus Trichinella is currently divided into seven species and at least three additional, unclassified genotypes, Trichinella T6, T8 and T9, where both T8 and T9 have been deemed very similar to T. britovi. Other than for the non-encapsulated species, the absence of distinguishing morphological characters and the overlapping nature of the biological characters within this genus make these tr...
متن کاملTrichinella according to their geographical distribution and biological, morphological and biochemical characteristics: Trichinella spiralis; Trichinella nativa; Trichinella britovi; Trichinella pseudospiralis; Trichinella murrelli; Trichinella nelsoni; Trichinella papuae;
The first outbreak of human trichinellosis in China,according to official national publication,was registered in Tibet in 1964. Since then,more than 500 outbreaks, numbering 25 161 cases with 240 deaths, have been recorded.However, this reported quantity is probably highly underestimated because adequate diagnostic techniques might not have been available in the whole area.Moreover, infection i...
متن کاملMolecular identification of Korean Trichinella isolates.
Muscle larvae of Trichinella isolates from two outbreaks in Korea were analyzed by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and multiple-PCR. All of the muscle larvae showed a band similar to that of T. spiralis larvae of the reference strain. The two Korean Trichinella isolates (isolate code ISS623 and ISS1078) might be classifiable to Trichinella spiralis.
متن کاملTrichinella murrelli in scavenging mammals from south-central Wisconsin, USA.
Tissues and serum from 59 raccoons (Procyon lotor), 42 coyotes (Canis latrans), and seven Striped Skunks (Mephitis mephitis) collected in Dane and Iowa Counties, Wisconsin, USA, between October 2005 and March 2006 were microscopically and serologically examined for the presence of Trichinella spp. Encapsulated larvae were found on compression slides prepared from tongue tissues from a few anima...
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ژورنال
عنوان ژورنال: Parasite
سال: 2017
ISSN: 1776-1042
DOI: 10.1051/parasite/2017053